作者簡介

Chao-Hung Lee

　　美國印地安那大學醫學院微生物及免疫研究所博士。

• Chapter 1: Nucleic Acid Structure and Properties(第1頁)
  • 1.1: Nucleic Acid(第1頁)
  • 1.2: Ribose and Base(第2頁)
  • 1.3: Nucleoside(第4頁)
  • 1.4: Nucleotide(第4頁)
  • 1.5: Structure of DNA and RNA(第6頁)
  • 1.6: Physical Properties of DNA and RNA(第11頁)
• Chapter 2: Replication， Transcription and Translation(第17頁)
  • 2.1: Introduction(第17頁)
  • 2.2: DNA Replication(第18頁)
  • 2.3: Transcription 20(第20頁)
  • 2.4: Capping of Pre- mRNA(第21頁)
  • 2.5: Splicing of Pre- mRNA(第22頁)
  • 2.6: Polyadenylation of Pre- mRNA(第27頁)
  • 2.7: Translation(第28頁)
  • 2.8: Regulation of Translation(第32頁)
  • 2.9: Ribosome(第34頁)
  • 2.10: Translation Factors(第36頁)
  • 2.11: Eukaryotic Translation(第38頁)
  • 2.12: Internal Ribosome Entry Site(第40頁)
• Chapter 3: Isolation of DNA and RNA(第49頁)
  • 3.1: Plasmid DNA Isolation(第49頁)
  • 3.2: Genomic DNA Isolation(第54頁)
  • 3.3: Total RNA Isolation(第55頁)
  • 3.4: Isolation of Poly-A+ mRNA(第56頁)
  • 3.5: Determination of the Quality and Quantity of DNA or RNA(第57頁)
• Chapter 4: Enzymes Used in Molecular Biology(第61頁)
  • 4.1: Types of Enzymes used in Molecular Biology(第61頁)
  • 4.2: Degradation Enzymes(第62頁)
  • 4.3: Discovery of Restriction Enzymes(第63頁)
  • 4.4: Classifi cation of Restriction-Modifi cation Enzymes(第65頁)
  • 4.5: Nomenclature of Restriction Enzymes(第67頁)
  • 4.6: DNA Ends Generated by Restriction Enzymes(第67頁)
  • 4.7: Factors Infl uencing the Activity of Restriction Enzymes(第68頁)
  • 4.8: Common Restriction Enzymes(第70頁)
  • 4.9. Isoschizomers(第71頁)
  • 4.10: Methyltransferases(第73頁)
  • 4.11: Other Degradation Enzymes(第75頁)
  • 4.12: RNA Polymerases(第77頁)
  • 4.13: DNA Polymerases(第77頁)
  • 4.14: Modifi cation Enzymes(第79頁)
• Chapter 5: Promoter and Operon(第85頁)
  • 5.1: Promoter(第85頁)
  • 5.2: Transcription Terminators of Prokaryotic Genes(第88頁)
  • 5.3: Eukaryotic Gene Transcription(第90頁)
  • 5.4: Elements Affecting Eukaryotic Gene Transcription(第91頁)
  • 5.5: Operon(第92頁)
  • 5.6: Lac Operon(第93頁)
  • 5.7: Suppression of Lac Operon(第94頁)
  • 5.8: Activation of Lac Operon(第96頁)
Chapter 6: Electrophoresis(第103頁)
• 6.1: Types of Electrophoresis(第103頁)
• 6.2: Electrophoresis of RNA(第104頁)
• 6.3: Resolution limits of Electrophoresis Gels(第105頁)
• 6.4: Pulsed-fi eld Gel Electrophoresis(第105頁)
• 6.5: Preparation of DNA Samples for PFGE(第107頁)
• 6.6: Factors Affecting the Effi ciency of PFGE(第107頁)
Chapter 7: Cloning(第111頁)
• 7.1: Requi red Elements of Cloning Vectors(第111頁)
• 7.2: pBR322 and pUC19 Vectors(第113頁)
• 7.3: Isolation of DNA Fragments for Cloning(第116頁)
• 7.4: Determination of Quantity and Quality of DNA Fragments(第117頁)
• 7.5: Determination of Insert to Vector Ratio(第118頁)
• 7.6: Spin Dialysis(第118頁)
• 7.7: Transformation(第120頁)
• 7.8: Screening of Transformants(第121頁)
• 7.9: Ligation of DNA Fragments with Vector(第123頁)
• 7.10: Prevention of Vector Self Ligation(第127頁)
• 7.11: Use of Tailing， Adaptors and Linkers(第128頁)
• 7.12: E. coli Genotypes(第129頁)
Chapter 8: Nucleic Acid Hybridization(第139頁)
• 8.1: Defi nition and Application of Nucleic Acid Hybridization(第139頁)
• 8.2: Southern Hybridization(第141頁)
• 8.3: Probe Labeling(第143頁)
• 8.4: Preparation of RNA Probes(第144頁)
• 8.5: Non-radioactive Probes(第146頁)
• 8.6: Pre hybridization(第151頁)
• 8.7: Stringency of Hybridization(第151頁)
• 8.8: Northern Hybridization(第153頁)
• 8.9: Types of Probes(第153頁)
Chapter 9: Library(第161頁)
• 9.1: Definition and Types of Library(第161頁)
• 9.2: Life Cycle of Lambda Bacteriophage(第162頁)
• 9.3: Lambda Vectors(第165頁)
• 9.4: Transcription Regulation of Lambda Bacteriophage(第167頁)
• 9.5: Induction of Lambda Lysogen(第169頁)
• 9.6: Factors affecting the Life Cycle of Lambda Phage(第170頁)
• 9.7: Clear Mutants of Lambda Bacteriophage(第171頁)
• 9.8: Property and Application of λgt10(第172頁)
• 9.9: Property and Application of λgt11(第172頁)
• 9.10: Generalized and Specialized Transducers(第173頁)
• 9.11: cDNA Synthesis(第174頁)
• 9.12. Packaging of Lambda Bacteriophage(第178頁)
• 9.13: In Vitro Lambda Packaging(第180頁)
• 9.14: Screening of cDNA Libraries(第182頁)
• 9.15: Unidirectional cDNA Cloning(第183頁)
• 9.16: Subtractive cDNA Library(第184頁)
• 9.17. Differential cDNA Library Screening(第185頁)
Chapter 10: Restriction Mapping(第191頁)
• 10.1: Verifi cation of Recombinant Plasmids(第191頁)
• 10.2: Identifi cation of Restriction Sites on a Plasmid(第193頁)
Chapter 11: DNA Sequencing(第197頁)
• 11.1: Principles of DNA Sequencing(第197頁)
• 11.2: Maxam and Gilbert DNA Sequencing Method(第198頁)
• 11.3: Sanger’s DNA Sequencing Method(第201頁)
• 11.4: DNA labeling in Sanger’s Sequencing Method(第204頁)
• 11.5: Properties of M13 Bacteriophage(第207頁)
• 11.6. Development of M13 Vectors(第209頁)
• 11.7: Development of Phagemids(第211頁)
• 11.8: Common Sequencing Problems(第215頁)
• 11.9: Cycle Sequencing(第216頁)
• 11.10: Automated DNA Sequencing(第217頁)
• 11.11: Pyrosequencing(第218頁)
• 11.12: In vivo Excision of pBluescript from Lambda ZAP(第220頁)
Chapter 12: Cloning Vectors(第229頁)
• 12.1: Cloning Potential of Various Vectors(第229頁)
• 12.2: EMBL3 and EMBL4 Vectors(第230頁)
• 12.3: Cosmid(第232頁)
• 12.4: Yeast Artifi cial Chromosome(第234頁)
• 12.5: Bacterial Artifi cial Chromosome(第240頁)
• 12.6: P1 Artifi cial Chromosome(第242頁)
Chapter 13: Polymerase Chain Reaction(第249頁)
• 13.1: Invention of Polymerase Chain Reaction(第249頁)
• 13.2: PCR Primer Design(第252頁)
• 13.3: Types of PCR(第257頁)
• 13.4: PCR Contamination Problem(第263頁)
• 13.5: PCR Controls(第264頁)
• 13.6. Prevention of Non-specifi c PCR(第265頁)
• 13.7: PCR Applications(第266頁)
• 13.8: Cloning of PCR Products(第269頁)
• 13.9: Other DNA Polymerases for PCR(第271頁)
• 13.10: Other Methods for Nucleic Acid Amplifi cation(第272頁)
Chapter 14: Expression of Recombinant Proteins(第289頁)
• 14.1: Expression of Foreign Proteins in E. coli(第289頁)
• 14.2: Inducible Promoters(第290頁)
• 14.3: Fusion Proteins(第291頁)
• 14.4: Isolation and Purifi cation of Recombinant Proteins(第293頁)
• 14.5: Removal of Tags(第294頁)
• 14.6: In-frame Fusion(第295頁)
• 14.7: Construction of Expression Plasmids(第296頁)
• 14.8: Detection of Expressed Proteins(第299頁)
• 14.9: Phage Display(第301頁)
• 14.10: Eukaryotic Expression Systems(第306頁)
• 14.11: Regulation of Expression in Mammalian Cells(第315頁)
• 14.12: Introduction of Recombinant Plasmid into Eukaryotic Cells(第317頁)
• 14.13: Gateway Expression System(第319頁)
Chapter 15: Mutagenesis， Transgenic Animals and RNA Interference(第329頁)
• 15.1: Purpose of Mutagenesis(第329頁)
• 15.2: Site-directed Mutagenesis(第330頁)
• 15.3: Single-base Mutation(第330頁)
• 15.4: Insertion and Deletion by PCR(第333頁)
• 15.5: Advanced Methods for PCR Mutagenesis(第335頁)
• 15.6: Homologous Recombination(第339頁)
• 15.7: Insertion Mutagenesis by Homologous Recombination(第340頁)
• 15.8: Single and Double Crossing Over(第342頁)
• 15.9: Deletion Mutagenesis by Homologous Recombination(第344頁)
• 15.10: Gene Replacement by Homologous Recombination(第345頁)
• 15.11: Transgenic Animals(第347頁)
• 15.12: RNA Interference(第350頁)
Chapter 16: Viral Vectors(第357頁)
• 16.1: Elements Requi red for Viral Vectors(第357頁)
• 16.2: Retroviral Vectors(第358頁)
• 16.3: Lentiviral vectors(第362頁)
• 16.4: Adenovirus Vectors(第365頁)
• 16.5: Adeno-associated Viral Vectors(第369頁)
• 16.6: Herpes Simplex Virus Vector(第371頁)
Chapter 17: Special Techniques(第379頁)
• 17.1: Nuclear Run-on Assay(第379頁)
• 17.2: Reporter Assay(第380頁)
• 17.3: Electrophoretic Mobility Shift Assay(第381頁)
• 17.4: DNase I Footprinting(第382頁)
• 17.5: Chromatin Immunoprecipitation(第384頁)
• 17.6: Two-hybrid System(第385頁)
• 17.7: Three Hybrid System(第387頁)
• 17.8: Primer Extension for Determination of Transcription Start Site(第389頁)
• 17.9: S1 Nuclease Analysis(第390頁)
• 17.10: RNase A Protection Assay(第391頁)
• 17.11: Single Strand Conformation Polymorphism(第391頁)
• 17.12: Heteroduplex Assay(第392頁)
• 17.13: Denatu red Gradient Gel Electrophoresis(第392頁)
• 17.14: Random Amplifi cation of Polymorphic DNA or Arbitrary Primed(第393頁)
• 17.15: Amplifi ed Fragment Length Polymorphism(第393頁)
• 17.16: mRNA Differential Display(第395頁)
• 17.17: DNA Microarray(第396頁)
• 17.18: Serial Analysis of Gene Expression(第396頁)
• 17.19: Massively Parallel Signature Sequencing(第399頁)
• 17.20: Sequencing by Oligo nucleotide Ligation and Detection(第406頁)
• 17.21: Total Gene Expression Analysis(第413頁)
• 17.22: Representational Difference Analysis(第415頁)
Glossar(第421頁)
Index(第459頁)